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Amazon’s desire to power your television-viewing experience through its array of Fire TV devices took another step forward on Wednesday with the announcement of a new smart soundbar, a second-generation Fire TV Cube and more.

Amazon’s Prime membership program may have as many as 50 million members worldwide, RBC analyst Mark Mahaney said in a note to investors. According to a report by Re/code, Mahaney estimated that the company has between 30 million and 40 million Prime subscribers in the U.S., and a total of 40 million to 50 million worldwide.

Americans for Free Trade, a coalition of 150 trade association members involving multiple industries, was formed in September 2018 with an aim to oppose tariffs and highlight the benefits of international trade to the US economy.

Among the 500 genes active only in X-bearing sperm, 18 genes are tasked with encoding receptors, which can be potentially used to manipulate the sperm, according to the study.

Among Chinese respondents, those who live in third-and fourth-tier cities scored 77.7, higher than major city residents, indicating a significant spending demand emerging from smaller Chinese cities, the report found.